: The device typically ships with GD-1 glass capillaries (with filament), a spare heater, and a power supply cable.
Adjust the heater knob to a value (typically between 4-7, depending on glass type).
: Press the START button to begin the automated pull. Protocol Tips for Common Needs Desired Pipette Type Suggested Settings (Reference Only) Long, Thin Injection Needles High heating value + One-stage pulling Firm Patch-Clamp Electrodes Low heating value + Two-stage pulling Flexible, Sharp Tips Use all available weights to increase pull speed Gradual, Parallel Taper Use fewer weights (e.g., 2 Type Light) Maintenance and Troubleshooting NARISHIGE WEB NEWS Setting Values in the PC-10 Puller - NARISHIGE WEB NEWS
| Problem | Probable Cause | Remedy | | :--- | :--- | :--- | | Even if power is on, "HEATER LEVEL" does not display. | AC Power Cord is disconnected; voltage setting is incorrect; fuse is blown. | Plug cord in firmly; make correct voltage setting; check and replace fuse. | | Heater value is the same, but the pipette is different. | Heating Element is deteriorated. | Increase the heater value to compensate for loss of heat generation. | | Pipette looks distorted and off-center. | Glass capillary is not centered in the Heating Element. | Be sure the glass capillary is placed right at the center of the Heating Element. | | Heating function is inconsistent. | Heater Unit is affected by wind from AC, room temperature, and humidity. | Please place the instrument to avoid those factors as much as possible. | | The heating function is inconsistent and coils are touching. | The coils of Heating Element are in contact. | Use a wrench to separate them, or replace it with a new unit of PC-10H. |
Proper installation is critical to ensure safety and prevent environmental factors from interfering with pipette reproducibility. Environmental Requirements Place the unit on a heavy, vibration-free laboratory bench. narishige pc10 manual new
The original manuals? They’ve become ghosts.
Available in 100V, 120V, or 220-240V AC configurations (verify your local voltage matching the rear label). 2. Installation and Safety Setup Environmental Requirements
Mastering the PC-10 involves balancing its key parameters. Understanding how to use these controls is the first step to producing perfect pipettes.
Yet, when a lab inherits a PC-10 today—often bought second-hand from an online auction or passed down by a retiring professor—the manual is almost always missing. And without it, the machine is a silent metal box with cryptic knobs labeled HEATER , MAGNET , and TIMER . : The device typically ships with GD-1 glass
The Narishige PC-10 is a dual-stage vertical micropipette puller widely recognized for its reliability in producing consistent glass capillaries and microelectrodes. Whether you have recently acquired a new unit, upgraded your laboratory infrastructure, or are looking to train new laboratory personnel, understanding the operational mechanics of the PC-10 is essential for achieving optimal experimental reproducibility.
AC 100V, 120V, 220V, or 240V (factory configured), 50/60Hz. Box Contents Checklist PC-10 Main Puller Unit Acrylic Dust Cover Replacement Platinum Heating Coils (typically 2 units) Standard Weights (for adjusting pull tension) Power Cord and Instruction Sheet 2. Setup and Installation
A key feature that set the PC-10 apart was its two pulling modes: a single-pull and a double-pull. The single-pull mode pulls the glass in one continuous stretch, ideal for longer, thinner needles. The double-pull mode, however, is where the PC-10’s versatility shines. It allows the heating and pulling parameters to be changed mid-process, enabling researchers to produce both long, thin needles for microinjection and robust microelectrodes for patch clamping on the same device. To adjust the pulling tension, the unit comes with four weights (two light and two heavy) that can be attached or removed to fine-tune the gravitational force.
That said, if you have an original (analog, two‑heater, gravity‑based puller), I can provide a comprehensive operational guide based on the original manual and common lab practices. Protocol Tips for Common Needs Desired Pipette Type
The unit utilizes a main slider assembly. To pull fine-tipped patch-clamping pipettes, you may need to add or subtract the removable ring weights included in the accessory kit. Installing the Heating Coil
You will also need to order consumable glass capillaries. The compatible glass types include:
When setting up a new Narishige PC-10, proper installation ensures mechanical calibration remains intact and prevents premature wear on the heating filament. Environmental Requirements
Ensure the glass is clamped symmetrically across the coil center. 6. Maintenance and Cleaning