Load tubes symmetrically in the rotor. The 5402 requires balanced loads.
The unit accepts various rotors to accommodate smaller tube sizes like 250 µl, 400 µl, and 500 µl, though these often require specific adapters .
: The unit includes an imbalance sensor and an automatic lid lock that prevents opening while the rotor is spinning. National Institute of Standards and Technology (.gov) Troubleshooting & Maintenance
Follow the updated maintenance guidelines to ensure the centrifuge complies with safety standards.
Shows the set speed or current running speed. eppendorf 5402 manual updated
Wipe down the inner rotor chamber with a damp cloth and mild detergent.
To serve as a practical supplement to the original Eppendorf 5402 instruction manual (cat. no. 5402 000.010). This guide incorporates current safety standards, rotor care protocols, and troubleshooting insights not detailed in the original 1990s publication.
Set the speed (RPM or RCF/g-force) and time using the control knob. Start: Close the main lid and press the START key.
The Eppendorf 5402 manual is a comprehensive guide that provides detailed information on the instrument's operation, maintenance, and troubleshooting. The manual covers the following topics: Load tubes symmetrically in the rotor
: Relates to rotor recognition. Ensure a compatible rotor is installed and try power-cycling the device.
Activates the refrigeration compressor at maximum capacity to chill the rotor chamber before a run.
Turn off the unit. Allow the motor to cool down for 30 minutes before restarting. Condenser coils blocked or compressor failure.
If your Eppendorf 5402 encounters issues, refer to this troubleshooting table: Potential Cause Power cable not connected Ensure proper electrical connection. Check fuse. Lid Does Not Lock Lid not shut properly Press down on the lid and try again. Ensure no obstruction. Error Code (Imbalance) Samples not balanced Stop, redistribute samples properly, and restart. Cooling Insufficient Dust buildup on condenser Vacuum the ventilation vents. Lid Won't Open Power failure or error Use the manual override release (see manual). : The unit includes an imbalance sensor and
Always load tubes symmetrically across the center axis of the rotor.
No rotor detected; ensure the rotor is properly seated and secured .
The Eppendorf 5402 offers several benefits to laboratory professionals, including: